christopherseiler

 For whole blood samples, we only recommend the use of this tube. BD PAXgene Blood RNA Tube (Item No.762165) 02 Collect blood Please read the product instructions carefully before using the PAXgene blood RNA tube for correct operation. If the PAXgene Blood RNA tube is the only blood drawing tube, the blood should be drawn into a "waste tube" prior to being drawn into the PAXgene Blood RNA tube so that the phlebotomizer used in the blood drawing process can be prefilled with blood; otherwise,jacketed glass reactor, the PAXgen Blood RNA tube should be the last tube in the blood drawing procedure. Make sure that the blood has stopped flowing into the tube before removing the tube from the needle holder (the negative pressure in the PAXgene Blood RNA tube is designed to draw 2.5ml of blood into the tube). 01 Selection of blood collection tube For serum samples, it is enough to use common serum vacuum blood collection tubes; for plasma samples,winterization filtration, it is recommended to use EDTA anticoagulant tubes, and heparin anticoagulant tubes are prohibited. 02 Separation and storage of plasma samples Collect blood samples with EDTA anticoagulant tube (the collected blood samples can be stored at room temperature or 4 ℃ for a short time, but not more than 1 H). The exosomes sample solution was stored at -80 ° C. Bulk dry ice transport. 5 Tissue samples For oral tissue samples used for RNA extraction, if the sample volume is small, it is recommended to give priority to the sample delivery method of protective solution (RNAlater, cbd centrifugal extractor ,jacketed glass reactor, etc.). Scheme I. Sample delivery of protective solution (RNAlater, etc.) The fresh tissue was cut into samples with length, width and height ≤ 0.5 cm with a scalpel, and small organs such as mouse liver, kidney and spleen could be preserved intact in RNA later. Immerse the tissue sample in 5-10 times the volume of the RNAlater to completely submerge the tissue. Samples were incubated overnight at 4 ° C (to allow complete tissue penetration by RNAlater) and then transferred to -80 ° C for long-term storage. Bulk dry ice transport. Scheme II:  1 X 10 ^ 7, and the number of cells required for oned do not make the cell centrifugation too solid to cause the lysate can not fully penetrate) to obtain cell sediment, discard PBS, add appropriate amount of TRIzol, and repeatedly suck and beat for several times until no agglomerated cell mass can be seen, so that it can be fully dissolved in the lysate. It is stored at -80 ℃ for a long time and transported in large volume of dry ice. Caution: 1. If it is DNA items can be washed with ordinary sterile PBS. If it is RNA items, it must be RNA se-free. 2. Trypsin treatment is not recommended for obtaining cell Pancreatin is protein, and if there is any residue, it will affect subsequent experiments. 3. The number of cells is less than 5 × 105 It is recommended to send the sample directly after quick freezing (without lysis with lysis solution). Due to the small number of cells,wiped film distillation, we will arrange micro-extraction. 4. Cell samples are not recommended to be stored in RNAlater tissue RNA protection solution, because of the high density of RNAlater, the cells stored in it are difficult to be collected by centrifugation for extraction. Return to Sohu to see more Responsible Editor:. toptiontech.com